Bovine IgG ELISA Kit


Product Number: ELISA-BIG-001

Pack Size

1 x 96 well Plate

Product Description

Designed for diagnostic purposes, the Biopanda Bovine IgG ELISA kit with high sensitivity is based on a sandwich enzyme-linked immunosorbent assay (ELISA) for measuring IgG in bovine biological specimens.

Principle

The test system for the detection of IgG in bovine biological specimens is designed in the format of a double antibody sandwich ELISA. In this test system the IgG present in specimens, will bind onto the wells of a microtitre plate (strip) supplied and pre-coated with anti-IgG antibodies. The bound IgG is detected by using horseradish peroxidase (HRP) conjugated anti-IgG antibodies, which in turn is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The quantity of bound HRP varies directly with the concentration of IgG in the test specimen; thus the absorbance at 450nm is a measure of the concentration of IgG in the test specimen. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards and corrected for specimen dilution.

Storage and Stability

  • The kit should be stored at 2–8°C. Do not freeze.
  • Unused test wells should be sealed and stored at 2–8°C.
  • This kit is valid until the expiration date printed on the label.

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