Bovine Transferrin ELISA Test Kit New Product


Product Number: ELISA-BTF-001

Pack Size

1 x 96 tests

Product Description

The Biopanda Bovine Transferrin ELISA Test kit is a double sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of total transferrin in bovine biological samples. Transferrin is a glycoprotein, mainly produced in the liver that binds and transports iron to bone marrow and tissue organs such as the liver.

Principle

The test kit for the detection of transferrin in bovine biological samples is designed in the format of a double sandwich ELISA. In this test system the transferrin present in specimens will bind onto the wells of the microtiter plates supplied and pre-coated with bovine transferrin antibodies. The bound transferrin is detected by using horseradish peroxidise (HRP) conjugated bovine transferrin antibodies, which in turn is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The quantity of bound HRP varies directly with the concentration of tranferrin in the tested specimen; thus, the absorbance, at 450nm, is a measure of the concentration of transferrin in the test specimen. The quantity of transferrin in the test specimen can be interpolated from the standard curve constructed from the standards and corrected for specimen dilution.

  • Method: Microtiter-based ELISA, in a breakaway microwell format (12 x 8 wells)
  • Type of sample: Serum
  • Conjugate: Horseradish peroxidase at working dilution (ready-to-use)
  • Substrate: TMB (tetramethylbenzidine) (single component) (ready-to-use)
  • Test runtime: less than 60 minutes
  • Shelf life: at least 1 year from manufacture when stored at 2°C - 8°C

Storage Conditions

+ 2 - 8 °C


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