ELISA Test Kits

IBR ELISA Test kit New Product

Product Number: ELISA-IBR-001

Pack Size

1 x 96 tests

Intended Use

The Biopanda Infectious Bovine Rhinotracheitis (IBR) test kit is a highly sensitive indirect ELISA for the screening of antibodies to Bovine herpesvirus-1 (BHV-1) in bovine blood, serum, and milk samples.

Principle of the Assay

The test kit for the detection of antibodies to Bovine herpesvirus-1 in bovine blood, serum, and milk samples is designed in the format of an indirect ELISA. Antibodies against BHV-1 which are present in the serum sample will bind onto the wells of the microtitre plate supplied precoated with BHV-1 antigen. The bound antibodies are detected by using horseradish peroxidase (HRP) conjugated monoclonal anti-bovine HBV-1 antibodies, which in turn is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The colour intensity of the solution depends on the antibody concentration in the test sample.

The features of the test are as followed:

  • Method: Microtiter-based ELISA (12 x 8 wells)
  • Type of sample: Bovine blood, serum, and milk
  • Sample volume: 10 µl per well
  • Substrate: TMB (tetramethylbenzidine) (single component) (ready-to-use)
  • Test overrun time: around 2 hours
  • Shelf life: 15 months from manufacture when stored at 2°C - 8°C

The ELISA kit is a non-radioactive, rapid assay that can be completed within 2 hours using simple colorimetric detection. The kit includes pre-coated and pre-blocked stabilised 96-well plate sealed in vacuum bags.

Storage Conditions

+ 2 - 8 °C

Product Image(s):

IBR ELISA Test kit

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