Vitamin D Rapid Test CE Marking

Product Number: RAPG-VTD-001

vitamin d rapid test cassette

Pack size

20 tests

Product Description

The Biopanda Vitamin D Rapid Test Semi-quantitatively detects 25-hydroxyvitamin D (25 (OH) D) in human fingerstickWhole blood at a cut-off concentration of 30 ± 4ng/ml. This assay provides a preliminary diagnostic test result and can be used for the screening for Vitamin D deficiency.


The Biopanda Vitamin D Rapid test is an immunoassay based on the principle of competitive binding. During testing, the mixture migrates laterally along the membrane chromatographically by capillary action. The membrane is pre-coated with 25 (OH) D antigen on the test line region of the strip. During testing, 25 (OH) D present in the specimen will compete with 25 (OH) D on the test line for limited amount of anti-25 OH Vitamin D antibodies in the conjugate. The higher concentration of 25 (OH) D in the specimen, the lighter shade of colour would be on the T line. The result will be read according to colour card provided with the kit.
To serve as a procedural control, a coloured line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

Test Attributes

  • Whole Blood, Serum or Plasma samples may be used for testing
  • Results available in only 10 minutes
  • Easy to use
  • Results can be read visually
  • No need for an analyser
  • Cost effective method for assisting in the diagnosis of Vitamin D deficiency

Test Procedure

  1. Ensure the test kit reagents and sample reach room temperature (15-30°C) prior to testing. Remove the test cassette from the sealed pouch and use it immediately.
  2. Place the cassette on a clean and level surface.
  3. Fill the capillary tube and transfer approximately 20 μl of fingerstick whole blood specimen to the specimen area of test cassette, then add 2 drops of buffer to the buffer area of the cassette and start the timer. See illustration below.
  4. Wait for the coloured line(s) to appear. Read results at 10 minutes by comparing the T line intensity with the provided colour card. Results read after 20 minutes are considered invalid.

Performance Characteristics

  • Overall Accuracy: 93.8%

Storage Conditions

Store the kit between 2-30°C and ensure the kits are not frozen or stored in direct sunlight. The test is valid until the expiration date printed on the foil wrapping.

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