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FOB and Transferrin Rapid Test CE Marking


Product Number: RAPG-FOT-001

Pack size

10 tests

Product Description

The Biopanda Faecal Occult Blood (FOB) and Transferrin Rapid Test detects the presence of human faecal occult blood and transferrin in human faecal samples. This test is an aid for the diagnosis of gastrointestinal bleeding disorders.

Test Attributes

  • Faecal samples may be used for testing
  • Results available in only 5 minutes
  • Easy to use
  • Results can be read visually
  • No need for an analyser
  • Cost effective method for assisting in the detection of gastrointestinal bleeding disorders

Test Procedure

  1. To collect faecal samples:
    Collect faeces in a clean, dry specimen collection container. Best results will be obtained if the assay is performed within 6 hours after collection. Specimens collected may be stored for 3 days at 2-8°C if not tested within 6 hours.
  2. To process faecal samples:
    Unscrew the cap of the specimen collection tube, then randomly stab the specimen collection applicator into the faecal specimen in at least 3 different sites. Do not scoop the faecal specimen.
    Screw on and tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer. Specimens prepared in the specimen collection tube may be stored for 6 months at -20°C if not tested within 1 hour after preparation.
  3. Bring the foil pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.
  4. Hold the specimen collection tube upright and open the cap of the specimen collection tube. Invert the specimen collection tube and transfer 2 full drops of the extracted specimen (approximately 80 μl) to the specimen well of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well. See illustration below.
  5. Read results at 5 minutes. Results read after 10 minutes are considered invalid.

FOB Transferrin rapid test

Principle

The Biopanda FOB/Transferrin Rapid Test is a qualitative, lateral flow immunoassay for the detection of Human Occult Blood and Transferrin in faeces. The membrane is precoated with anti-haemoglobin antibody and anti-transferrin antibody on the test line regions. During testing, the specimen reacts with the particle coated with anti-hemoglobin antibody and/or anti-transferrin antibody. The mixture migrates laterally along the membrane chromatographically by capillary action to react with anti-haemoglobin antibody and/or anti-transferrin antibody on the membrane and generates a coloured line. The presence of this coloured line in the test line region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a coloured line will always appear in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.

Performance Characteristics

    FOB Rapid Test:
  • Relative sensitivity: >97.6%
  • Relative specificity: 99.3%
  • Accuracy: 98.9%

  • Transferrin
  • Relative Sensitivity: >98.9%  
  • Relative Specificity: 99.4%
  • Accuracy: 99.3%

Storage Conditions

Store the kit between 2-30°C and ensure the kits are not frozen or stored in direct sunlight. The test is valid until the expiration date printed on the foil wrapping.


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