Melamine ELISA Kit

Product Number: ELISA-MEL-001

Pack Size

1 x 96 well Plate

Product Description

The Biopanda Melamine kit is a direct enzyme-linked immunosorbent assay (ELISA) to quantitatively detect the presence of Melamine in samples of milk, milk powder and animal feed. Melamine is an organic base and a trimer of cyanamide, with a 1,3,5-triazine skeleton. Like cyanamide, it contains 67% nitrogen by mass and, if mixed with resins, has fire retardant properties due to its release of nitrogen gas when burned or charred, and has several other industrial uses. Melamine is also a metabolite of cyromazine, a pesticide. It is formed in the body of mammals that have ingested cyromazine. It has been reported that cyromazine can also be converted to melamine in plants. The United Nations' food standards body has set the maximum amount of melamine allowed in powdered infant formula to 1 mg/kg and the amount of the chemical allowed in other foods and animal feed to 2.5 mg/kg.

SampleDetection LimitRecovery Rate
0.1 ppm/0.08 ppm
Milk Powder
0.3 ppm
Animal Feed
0.3 ppm

Kit Components

  • 1 x Pre-coated microtiter plate (12 x 8 microwells)
  • 1 x Enzyme conjugate solution (6 ml)
  • 1 x Wash buffer concentrate (20×, 40 ml)
  • 1 x Sample dilution buffer concentrate (10×, 50 ml)
  • 6 x AHD standards (1.0 ml each) (0 ng/ml, 10 ng/ml, 25 ng/ml, 50 ng/ml, 150 ng/ml, 500 ng/ml)
  • 1 x TMB substrate solution (12 ml)
  • 1 x Stop solution (6 ml)
  • 1 x Package insert
  • 1 x Microplate sealer

Storage Conditions

+ 2 - 8 °C

Shelf life

12 months

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