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Aflatoxin B1 ELISA Kit


Product Number: ELISA-AFB1-001

Pack Size

1 x 96 well Plate

Product Description

The Biopanda Aflatoxin B1 ELISA quantitatively detects the presence of Aflatoxin B1 in animal feed, cereals, cooking oil, bread and other food samples. Aflatoxin B1 is produced by Aspergillus flavus and Aspergillus parasiticus. Aflatoxin B1 is a common contaminant in a variety of foods including peanuts, corn, and other grains; as well as animal feeds. Aflatoxin B1 is considered the most toxic aflatoxin and it is highly implicated in hepatocellular carcinoma in humans.

SampleDetection LimitRecovery Rate
Animal Feed
2.5 ppb
80-110%
Cereals
0.5 ppb
80-110%
Rice Flour
0.5 ppb
90-110%
Cooking Oil
1.5 ppb
70-100%
Soy Sauce
0.5 ppb
80-110%
Bread
2.5 ppb
90-110%
Cakes
2.5 ppb
90-120%

Principle

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of Aflatoxin B1. An unknown amount of Aflatoxin B1 present in the sample and the fixed amount of Aflatoxin B1 antigens pre-coated on the wells of microtiter plate/strips compete for the anti-Aflatoxin B1 antibodies, which in turn is detected with enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The absorbance value of the developed colour is inversely proportional to the amount of the Aflatoxin B1 in the sample. The quantity of Aflatoxin B1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Kit Components

  • 1 x Pre-coated Microtiter plate (12 x 8 microwells)
  • 1 x Aflatoxin B1 Enzyme conjugate (6 ml)
  • 1 x Aflatoxin B1 Antibody solution (6 ml)
  • 1 x Wash buffer concentrate (10×, 50 ml)
  • 1 x Sample Diluent concentrate (10×, 20 ml)
  • 6 x Aflatoxin B1 standards (1.0 ml/each) (0 ng/ml, 0.05 ng/ml, 0.15 ng/ml, 0.45 ng/ml, 1.35 ng/ml, 4.05 ng/ml)
  • 1 x TMB solution (12 ml)
  • 1 x Stop solution (14 ml)
  • 1 x Package insert
  • 1 x Microplate sealer

Storage Conditions

+ 2 - 8 °C

Shelf life

12 months


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