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ELISA Test Kits

Fertility


Prolactin ELISA Kit


Product Number: ELISA-PROL-001

Pack Size

1 x 96 well Plate

Intended Use:

The Biopanda Prolactin ELISA test kit provides a means for the quantitative determination of prolactin in human serum.

Principle of the test:

The test kit is designed in the format of a double antibody sandwich ELISA. In this test system, prolactin present in samples binds onto wells of the microtiter plate coated with anti-prolactin antibody. Bound prolactin is then detected by using a Horseradish Peroxidise (HRP)-conjugated mouse monoclonal anti-prolactin antibody and visualised by adding the substrate TMB which gives a blue coloured product. This blue colour can be measured spectrophotometrically at 450 nm following the addition of an acidic stop solution, which turns it yellow. The absorbance at 450 nm is a measure of the concentration of prolactin in the test sample since the quantity of bound HRP varies directly with the prolactin concentration which can be interpolated from a standard curve constructed using standards.

Materials and Components:

  • Antibody-coated microtiter wells, 96 wells.
  • Reference standard set, contains 0, 5, 20, 50, 100, and 200 ng/ml human prolactin, in liquid form ( ready to use ) or lyophilized form.
  • Enzyme Conjugate Reagent, 12 mL.
  • TMB Substrate , 12 mL.
  • Stop Solution , 12 mL.
  • Wash Buffer Concentrate(50X), 15mL

Storage:

Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. The shelf life is at least 2 years from manufacture when stored at 2-8°C. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-2 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.

Shelf life

18 months


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