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ELISA Test Kits

Fertility


Progesterone ELISA Kit


Product Number: ELISA-PROG-001

Pack Size

1 x 96 well Plate

Intended Use:

The Biopanda Progesterone ELISA test kit provides a means for the quantitative determination of progesterone in human serum or plasma.

Principle of the test:

The Biopanda progesterone ELISA is based on the principle of competitive binding between progesterone in the test specimen and a progesterone-HRP conjugate for a constant amount of rabbit anti-progesterone. Goat anti-rabbit IgG-coated wells are incubated with the samples, progesterone-HRP Conjugate and rabbit anti-progesterone antibody at room temperature. During the incubation, the fixed amount of progesterone-HRP conjugate competes with the endogenous progesterone in the sample for binding to the rabbit anti-progesterone antibody. Thus, if there is a high concentration of progesterone in the sample, then there will be a significant reduction in signal output of the assay as the amount of progesterone-HRP conjugate bound to the rabbit anti-progesterone antibody in the well decreases. Conversely, if there is little progesterone in the sample, there will be minimal reduction in signal. Unbound progesterone-HRP conjugate is then removed. A blue colour is formed when substrate TMB is added, which can be read spectrophotometrically at 450 nm. The intensity of the colour formed is proportional to the amount of enzyme present and is inversely proportional to the amount of unlabelled progesterone in the sample. A standard curve of absorbance against progesterone standard concentrations is plotted and the quantity of progesterone in the unknown sample is read off from the graph.

Materials and Components:

  • Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells.
  • Progesterone Reference Standards: 0, 0.5, 3.0, 10, 25 and 50 ng/mL. Liquids, 0.5 mL each, ready to use.
  • Rabbit Anti-Progesterone Reagent, 7 mL.
  • Progesterone-HRP Conjugate Concentrate (11x), 1.3 mL.
  • Progesterone-HRP Conjugate Diluent, 13 mL
  • TMB Substrate, 12 mL.
  • Stop Solution , 12 mL.
  • Wash Buffer Concentrate(50X),15 mL.

Storage:

Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. The shelf life is at least 2 years from manufacture when stored at 2-8°C. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.

Shelf life

18 months


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