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ELISA Test Kits

Fertility


LH ELISA Kit


Product Number: ELISA-LH-001

Pack Size

1 x 96 well Plate

Intended Use:

The Biopanda Luteinizing Hormone (LH) ELISA test kit provides a means for the quantitative determination of LH concentration in human serum.

Principle of the test:

The test kit is designed in the format of a double antibody sandwich ELISA. In this test system, LH present in samples binds onto wells of the microtiter plate coated with anti-LH antibody. Bound LH is then detected by using a Horseradish Peroxidise (HRP)-conjugated mouse monoclonal anti-LH antibody and visualised by adding the substrate TMB which gives a blue coloured product. This blue colour can be measured spectrophotometrically at 450 nm following the addition of an acidic stop solution which turns it yellow. The absorbance at 450 nm is a measure of the concentration of LH in the test sample since the quantity of bound HRP varies directly with the LH concentration which can be interpolated from constructing a standard curve using standards.

Materials and Components:

  • Antibody-coated microtiter plate with 96 wells.
  • Reference standard set, contains 0, 5.0, 20, 50, 100, and 200 mIU/ml.(WHO, 1st IRP, 68/40), lyophilized.
  • Enzyme Conjugate Reagent, 12 mL.
  • TMB Substrate , 12 mL.
  • Stop Solution , 12 mL.
  • Wash Buffer Concentrate (50X),15 mL

Storage:

Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. The shelf life is at least 2 years from manufacture when stored at 2-8°C. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-2 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.

Shelf life

18 months


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