ELISA Test Kits



Product Number: ELISA-FSH-001

Pack Size

1 x 96 well Plate

Intended Use:

The Biopanda Follicle Stimulating Hormone (FSH) ELISA test kit provides a means for the quantitative determination of FSH in human serum or plasma.

Principle of the test:

The Biopanda FSH Quantitative Test Kit is a sandwich ELISA in which the FSH in test samples bind to wells of the plate coated with a polyclonal anti-FSH antibody. The bound FSH is then detected by using the enzyme Horseradish Peroxidise (HRP) conjugated to a mouse monoclonal anti-FSH antibody and visualised by adding the substrate TMB which gives a blue coloured product. This blue colour can be measured spectrophotometrically at 450 nm following the addition of an acidic stop solution which turns it yellow. The absorbance at 450 nm is a measure of the concentration of FSH in the test sample since the quantity of bound HRP varies directly with the FSH concentration which can be interpolated from a standard curve constructed using standards.

Materials and Components:

  • Antibody-coated microtiter wells, 96 wells per bag.
  • Reference standard set, contains 0, 5, 20, 50, 100 and 200
  • MIU/mL (WHO, 2nd IRP, HMG) human FSH, in liquid form (ready to use) or lyophilized form.
  • Enzyme conjugate reagent, 12 mL.
  • TMB Substrate, 12 mL.
  • Stop Solution, 12 mL.
  • Wash Buffer Concentrate(50X),15mL


Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. The shelf life is at least 2 years from manufacture when stored at 2-8°C. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.

Shelf life

18 months

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