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Estradiol ELISA Kit


Product Number: ELISA-EST-001

Pack Size

1 x 96 well Plate

Intended Use:

The Biopanda Estradiol ELISA test kit provides a means for the quantitative determination of Estradiol (E2) in human serum or plasma.

Principle of the test:

The Biopanda Estradiol (E2) ELISA is based on the principle of competitive binding between E2 in the test specimen and E2-HRP conjugate for a constant amount of rabbit anti-Estradiol.

Goat anti-rabbit IgG-coated wells are incubated with the samples, Estradiol HRP Conjugate and rabbit anti-Estradiol reagent. During the incubation, the fixed amount of Estradiol HRP conjugate competes with the endogenous E2 in the sample for binding to the rabbit anti-Estradiol antibody. Thus if there is a high concentration of E2 in the sample, then there will be a significant reduction in signal output of the assay as the amount of Estradiol HRP conjugate bound to the rabbit anti-Estradiol in the well decreases. Conversely, if there is little E2 in the sample, there will be minimal reduction in signal. Unbound Estradiol HRP conjugate is then removed. A blue colour is formed when substrate TMB is added, which can be read spectrophotometrically at 450 nm. The intensity of the colour formed is proportional to the amount of enzyme present and is inversely proportional to the amount of unlabelled E2 in the sample. A standard curve of absorbance against E2 standard concentration is plotted and the quantity of E2 in the unknown sample is read from the graph.

Materials and Components:

  • Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells
  • Estradiol Reference Standards: 0, 10, 30, 100, 300 and 1000 Pg/ml Liquid, 0.50 mL each, ready to use.
  • Rabbit Anti-Estradiol Reagent , 7.0 mL
  • Estradiol-HRP Conjugate Reagent , 12 mL
  • Wash Buffer Concentrate (50X),15 mL
  • TMB Substrate , 12 mL.
  • Stop Solution , 12 mL.

Storage:

Unopened test kits should be stored between 2-8°C and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. The shelf life is at least 2 years from manufacture when stored at 2-8oC. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.

Shelf life

18 months


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